Autoclave Calibration Record｜121°C Sterilization Test with BI Verification

This record outlines the calibration of a laboratory autoclave (steam sterilizer), used for sterilizing glassware, media, and biological waste. Accurate sterilization is vital for lab safety and experiment integrity.

READY Biotech technicians performed the following validation steps:

▣ Empty / Full Load Sterilization Test

• Programmed sterilization at 121°C for 20 minutes, following standard lab SOP

• Performed under both empty and fully loaded conditions to simulate real use

• Standard thermometer probes recorded temperature throughout the cycle: heat-up, holding, and cool-down

▣ Biological Indicator (BI) Verification

• Commercial spore-based biological indicators were placed in critical locations

• After sterilization, BIs were incubated to confirm complete microbial inactivation

• Served as a biological validation of sterilization effectiveness

▣ Report and Traceability

• Calibration report includes time-temperature graphs and BI result documentation

• Ensures traceability for audits, quality control, and internal recordkeeping

Need to confirm your autoclave’s sterilization performance?
 Contact READY Biotech for professional on-site calibration and verification service!

☎︎ Contact: +886-2-8228-0131
✉︎ Email: Ready_lab@outlook.com