Autoclave Validation: Ensuring Reliable 121°C Sterilization

READY Biotech provides full autoclave validation including empty chamber heat distribution, load heat penetration, biological indicator (BI) test, pressure test, and 31-minute timer verification, with complete F0 values and temperature curve reports.

01｜Empty Chamber Heat Distribution

• Temperature sensors placed throughout the empty chamber

• Verifies uniform heating to 121°C

• Identifies cold spots that may reduce sterilization effectiveness

02｜Load Heat Penetration Test

• Performed with simulated or customer-specified load

• Confirms temperature penetration to the load’s core

• Ensures real-world sterilization reliability

03｜Biological Indicator (BI) Test

• Uses Geobacillus stearothermophilus spore indicators

• Post-cycle incubation and observation

• Pass/Fail determination based on microbial growth

• Full documentation with photos

04｜Pressure Test (Equipment Dependent)

Performed only when the autoclave model allows:

• Checks pressure rise and release behavior

• Compares actual vs. displayed pressure

• Identifies leaks or seal issues

05｜Timer Verification – 31 Minutes at 121°C

Once the autoclave display stabilizes at 121°C:

• Start both the built-in timer and a certified reference timer

• Compare the full 31-minute sterilization holding time

• Ensures accurate exposure time for the sterilization cycle

06｜Comprehensive Validation Report

Includes:

• Temperature curve graphs

• F0 value

• Heat distribution / penetration data

• BI test results

• Pressure test (if applicable)

• Timer verification result

☎︎ Tel: 02-8228-0131

✉️ Email: Ready_lab@outlook.com